J. Keith Joung, M.D., Ph.D.

Associate Chief of Pathology for Research
Associate Professor of Pathology
The Jim and Ann Orr MGH Research Scholar

Molecular Pathology Unit
Center for Computational and Integrative Biology
Center for Cancer Research

Massachusetts General Hospital
149 13th Street, 6th floor
Charlestown, MA 02129

E-mail: JJOUNG@PARTNERS.ORG


Education & Training:

1998-2001

Post-doctoral fellowship, Massachusetts Institute of Technology

1996

Ph.D. in Genetics, Harvard University

1996 M.D., Harvard Medical School
1987 A.B., Biochemical Sciences, Harvard College

Positions:

2009-pres

Associate Pathologist, Massachusetts General Hospital

2009-pres

Associate Chief of Pathology for Research, Massachusetts General Hospital

2008-pres Associate Professor of Pathology, Harvard Medical School
2007-pres. Director, Molecular Pathology Unit, Massachusetts General Hospital
2007-pres. Member, Center for Computational and Integrative Biology, Massachusetts General Hospital
2004-2008 Assistant Professor of Pathology, Harvard Medical School
2004-pres. Faculty member, Ph.D. program in Biological and Biomedical Sciences, Division of Medical Sciences, Harvard Medical School
2003-pres. Member, Center for Cancer Research, MGH Cancer Center
2000-2008 Assistant Pathologist, Massachusetts General Hospital
1998-2001 Post-doctoral Fellow, HHMI/Massachusetts Institute of Technology
1997 Chief Resident in Clinical Pathology, Massachusetts General Hospital
1996-1999 Resident in Clinical Pathology, Massachusetts General Hospital

Honors:

2011

Appointment as The Jim and Ann Orr MGH Research Scholar

2010

NIH Director's Pioneer Award recipient

2010

NIH Transformative R01 recipient

2009 Honoree, The One Hundred, MGH Cancer Center
2000-2005 NIH K08 fellowship award
2000 Board Certification in Clinical Pathology, American Board of Pathology
1998-2000 Howard Hughes Medical Institute Postdoctoral Research Fellowship for Physicians
1996 Bernard N. Fields Prize in Microbiology and Molecular Genetics (for Ph.D. thesis), Harvard Medical School
1989-1996 Medical Scientist Training Program (M.D.-Ph.D.) appointment, Harvard Medical School
1987 Graduated magna cum laude, Harvard College

Publications:

Pattanayak V, Ramirez CL, Joung JK, Liu DR. Revealing off-target cleavage specificities of zinc-finger nucleases by in vitro selection. Nat Methods. 2011 Aug 7. doi: 10.1038/nmeth.1670. [Epub ahead of print]

Sander JD, Cade L, Khayter C, Reyon D, Peterson RT, Joung JK*, Yeh JR*. Targeted gene disruption in somatic zebrafish cells using engineered TALENs. Nat Biotechnol. 2011 Aug 5;29(8):697-8.
*Co-corresponding authors

Lee J, Hirsh AS, Wittner BS, Maeder ML, Singavarapu R, Lang M, Janarthanan S, McDermott U, Yajnik V, Ramaswamy S, Joung JK*, Sgroi DC*. Induction of stable drug resistance in human breast cancer cells using a combinatorial zinc finger transcription factor library. PLoS One. 2011;6(7):e21112.
*Co-corresponding authors

Rahman SH, Maeder ML, Joung JK, Cathomen T. Zinc-finger nucleases for somatic gene therapy: the next frontier. Hum Gene Ther. 2011 Jun 1. [Epub ahead of print]

Curtin SJ, Zhang F, Sander JD, Haun WJ, Starker C, Baltes NJ, Reyon D, Dahlborg EJ, Goodwin MJ, Coffman AP, Dobbs D, Joung JK, Voytas DF, Stupar RM. Targeted mutagenesis of duplicated genes in soybean with zinc-finger nucleases breakthrough technologies. Plant Physiol. 2011 Jun;156(2):466-73.

Reyon D, Kirkpatrick JR, Sander JD, Zhang F, Voytas DF, Joung JK, Dobbs D, Coffman CR. ZFNGenome: a comprehensive resource for locating zinc finger nuclease target sites in model organisms. BMC Genomics. 2011 Jan 28;12:83.

Sander JD, Dahlborg EJ, Goodwin MJ, Cade L, Zhang F, Cifuentes D, Curtin SJ, Blackburn JS, Thibodeau-Beganny S, Qi Y, Pierick CJ, Hoffman E, Maeder ML, Khayter C, Reyon D, Dobbs D, Langenau DM, Stupar RM, Giraldez AJ, Voytas DF, Peterson RT, Yeh JR, Joung JK. Selection-free zinc-finger-nuclease engineering by context-dependent assembly (CoDA). Nat Methods. 2010 Dec 12; doi: 10.1038/nmeth.1542.

Sander JD, Reyon D, Maeder ML, Foley JE, Thibodeau-Beganny S, Li X, Regan MR, Dahlborg EJ, Goodwin MJ, Fu F, Voytas DF, Joung JK, Dobbs D. Predicting success of oligomerized pool engineering (OPEN) for zinc finger target site sequences. BMC Bioinformatics. 2010; 11: 543.

Söllü C, Pars K, Cornu TI, Thibodeau-Beganny S, Maeder ML, Joung JK, Heilbronn R, Cathomen T. Autonomous zinc-finger nuclease pairs for targeted chromosomal deletion. Nucleic Acids Res. 2010 Aug 16. Epub ahead of print.

Sander JD, Maeder ML, Reyon D, Voytas DF, Joung JK, Dobbs D. ZiFiT (Zinc Finger Targeter): an updated zinc finger engineering tool. Nucleic Acids Res. 2010 Jul 1;38 Suppl:W462-8.

Zhang F, Maeder ML, Unger-Wallace E, Hoshaw JP, Reyon D, Christian M, Li X, Pierick CJ, Dobbs D, Peterson T, Joung JK, Voytas DF. High frequency targeted mutagenesis in Arabidopsis thaliana using zinc finger nucleases. Proc Natl Acad Sci U S A. 2010 Jun 29;107(26):12028-33.

Thibodeau-Beganny S, Maeder ML, Joung JK. Engineering single Cys2His2 zinc finger domains using a bacterial cell-based two-hybrid selection system. Methods Mol Biol. 2010;649:31-50.

Joung JK, Voytas DF, Cathomen T. Reply to "Genome editing with modularly assembled zinc-finger nucleases," Nat Methods. 2010, 7: 91-92.

Voytas DF, Joung JK. Plant science. DNA binding made easy. Science. 2009 Dec 11;326(5959):1491-2.

Zou J, Maeder ML, Mali P, Pruett-Miller SM, Thibodeau-Beganny S, Chou BK, Chen G, Ye Z, Park IH, Daley GQ, Porteus MH, Joung JK, Cheng L. Gene targeting of a disease-related gene in human induced pluripotent stem and embryonic stem cells. Cell Stem Cell. 2009 Jul 2;5(1):97-110.

Townsend JA, Wright DA, Winfrey RJ, Fu F, Maeder ML, Joung JK, Voytas DF. High-frequency modification of plant genes using engineered zinc-finger nucleases. Nature. 2009 May 21;459(7245):442-5.

Sander JD, Zaback P, Joung JK, Voytas DF, Dobbs D. An affinity-based scoring scheme for predicting DNA-binding activities of modularly assembled zinc-finger proteins. Nucleic Acids Res. 2009 Feb;37(2):506-15.

Foley JE, Maeder ML, Pearlberg J, Joung JK, Peterson RT, Yeh JR. Targeted mutagenesis in zebrafish using customized zinc-finger nucleases. Nat Protoc. 2009;4(12):1855-67.

Maeder ML, Thibodeau-Beganny S, Sander JD, Voytas DF, Joung JK. Oligomerized pool engineering (OPEN): an 'open-source' protocol for making customized zinc-finger arrays. Nat Protoc. 2009;4(10):1471-501.

Fu F, Sander JD, Maeder M, Thibodeau-Beganny S, Joung JK, Dobbs D, Miller L, Voytas DF. Zinc Finger Database (ZiFDB): a repository for information on C2H2 zinc fingers and engineered zinc-finger arrays. Nucleic Acids Res. 2009 Jan;37(Database issue):D279-83.

Foley JE, Yeh JR, Maeder ML, Reyon D, Sander JD, Peterson RT, Joung JK. Rapid mutation of endogenous zebrafish genes using zinc finger nucleases made by Oligomerized Pool ENgineering (OPEN). PLoS One. 2009;4(2):e4348.

Maeder ML, Thibodeau-Beganny S, Osiak A, Wright DA, Anthony RM, Eichtinger M, Jiang T, Foley JE, Winfrey RJ, Townsend JA, Unger-Wallace E, Sander JD, Müller-Lerch F, Fu F, Pearlberg J, Göbel C, Dassie JP, Pruett-Miller SM, Porteus MH, Sgroi DC, Iafrate AJ, Dobbs D, McCray PB Jr, Cathomen T, Voytas DF, Joung JK. Rapid "open-source" engineering of customized zinc-finger nucleases for highly efficient gene modification. Mol Cell. 2008 Jul 25;31(2):294-301.

Cathomen T, Joung JK. Zinc-finger nucleases: the next generation emerges. Mol Ther. 2008 Jul;16(7):1200-7.

Ramirez CL, Foley JE, Wright DA, Müller-Lerch F, Rahman SH, Cornu TI, Winfrey RJ, Sander JD, Fu F, Townsend JA, Cathomen T, Voytas DF, Joung JK. Unexpected failure rates for modular assembly of engineered zinc fingers. Nat Methods. 2008 May;5(5):374-5.

Pruett-Miller SM, Connelly JP, Maeder ML, Joung JK, Porteus MH. Comparison of zinc finger nucleases for use in gene targeting in mammalian cells. Mol Ther. 2008 Apr;16(4):707-17.

Cornu TI, Thibodeau-Beganny S, Guhl E, Alwin S, Eichtinger M, Joung JK, Cathomen T. DNA-binding specificity is a major determinant of the activity and toxicity of zinc-finger nucleases. Mol Ther. 2008 Feb;16(2):352-8.

Sander JD, Zaback P, Joung JK, Voytas DF, Dobbs D. Zinc Finger Targeter (ZiFiT): an engineered zinc finger/target site design tool. Nucleic Acids Res. 2007 Jul;35(Web Server issue):W599-605.

Thibodeau-Beganny S, Joung JK. Engineering Cys2His2 zinc finger domains using a bacterial cell-based two-hybrid selection system. Methods Mol Biol. 2007;408:317-34.

Meng X, Thibodeau-Beganny S, Jiang T, Joung JK, Wolfe SA. Profiling the DNA-binding specificities of engineered Cys2His2 zinc finger domains using a rapid cell-based method. Nucleic Acids Res. 2007;35(11):e81.

Meng X, Smith RM, Giesecke AV, Joung JK, Wolfe SA. Counter-selectable marker for bacterial-based interaction trap systems. Biotechniques. 2006 Feb;40(2):179-84.

Giesecke AV, Fang R, Joung JK. Synthetic protein-protein interaction domains created by shuffling Cys2His2 zinc-fingers. Mol Syst Biol. 2006;2:2006.2011.

Wright DA, Thibodeau-Beganny S, Sander JD, Winfrey RJ, Hirsh AS, Eichtinger M, Fu F, Porteus MH, Dobbs D, Voytas DF, Joung JK. Standardized reagents and protocols for engineering zinc finger nucleases by modular assembly. Nat Protoc. 2006;1(3):1637-52.

Vallet-Gely I, Donovan KE, Fang R, Joung JK, Dove SL. Repression of phase-variable cup gene expression by H-NS-like proteins in Pseudomonas aeruginosa. Proc Natl Acad Sci U S A. 2005 Aug 2;102(31):11082-7.

Serebriiskii IG, Fang R, Latypova E, Hopkins R, Vinson C, Joung JK, Golemis EA. A combined yeast/bacteria two-hybrid system: development and evaluation. Mol Cell Proteomics. 2005 Jun;4(6):819-26.

Nguyen-Hackley DH, Ramm E, Taylor CM, Joung JK, Dervan PB, Pabo CO. Allosteric inhibition of zinc-finger binding in the major groove of DNA by minor-groove binding ligands. Biochemistry. 2004 Apr 6;43(13):3880-90.

Thibodeau SA, Fang R, Joung JK. High-throughput beta-galactosidase assay for bacterial cell-based reporter systems. Biotechniques. 2004 Mar;36(3):410-5.

Thibodeau SA, Joung JK. An improved strategy for constructing "designer" Cys2His2 zinc finger proteins. Discov Med. 2003 Dec;3(19):32-5.

Hurt JA, Thibodeau SA, Hirsh AS, Pabo CO, Joung JK. Highly specific zinc finger proteins obtained by directed domain shuffling and cell-based selection. Proc Natl Acad Sci U S A. 2003 Oct 14;100(21):12271-6.

Joung JK. Identifying and modifying protein-DNA and protein-protein interactions using a bacterial two-hybrid selection system. J Cell Biochem. 2001;Suppl 37:53-7.

Joung JK, Ramm EI, Pabo CO. A bacterial two-hybrid selection system for studying protein-DNA and protein-protein interactions. Proc Natl Acad Sci U S A. 2000 Jun 20;97(13):7382-7.

Dove SL, Joung JK, Hochschild A. Activation of prokaryotic transcription through arbitrary protein-protein contacts. Nature. 1997 Apr 10;386(6625):627-30.

Joung JK, Chung EH, King G, Yu C, Hirsh AS, Hochschild A. Genetic strategy for analyzing specificity of dimer formation: Escherichia coli cyclic AMP receptor protein mutant altered in its dimerization specificity. Genes Dev. 1995 Dec 1;9(23):2986-96.

Joung JK, Koepp DM, Hochschild A. Synergistic activation of transcription by bacteriophage lambda cI protein and E. coli cAMP receptor protein. Science. 1994 Sep 23;265(5180):1863-6.

Joung JK, Le LU, Hochschild A. Synergistic activation of transcription by Escherichia coli cAMP receptor protein. Proc Natl Acad Sci U S A. 1993 Apr 1;90(7):3083-7.

Ruben S, Perkins A, Purcell R, Joung JK, Sia R, Burghoff R, Haseltine WA, Rosen CA. Structural and functional characterization of human immunodeficiency virus tat protein. J Virol. 1989 Jan;63(1):1-8.